This kit supplies the substrate and cell culture components to support a serum-free, feeder-free cell culture environment for experiments using induced pluripotent stem cells. The kit includes Pluripotent Stem Cell SFM XF/FF, Cell Basement Membrane, Stem Cell Dissociation Reagent, ROCK Inhibitor Y27632, Stem Cell Freezing Media, DMEM: F12 Medium, and D-PBS.